Authors : Z.H. Zhao, S.F. Li, H.Y. Huang, C.M. Li and J. Zhang
Abstract: Single Nucleotide Polymorphisms (SNPs) of Nuclear receptor Co-activator 1 gene (NCOA1), gene of shaobo breed were detected by using PCR-SSCP and sequencing reaction and its associations with semen quality was done. Three SNPs were detected, T/A substitution at position 10155007 in exon 3 and resulted in 3 genotypes AA, AB and BB, C/G substitution at position 108273257 in exon 10 and resulted in 3 genotypes CC, CD and DD, C/T substitution at position 108273423 in exon 12 and 3 genotypes of SS, ST and TT were found. AA genotype had more semen volume than BB (p<0.05) and it had significantly higher than AB at sperm density (p<0.05). No difference was found between CC, CD and DD (p>0.05) at semen volume but CC significantly higher than DD at sperm density (p<0.05). Sperm volume and sperm density among genotypes of SS, ST and TT were no significant significance (p>0.05). There were no significant differences at the sperm motility and the rate of abnormal sperm between all different genotype and haplotype (p>0.05). The haplotype effect of AACC was higher than the single genotype effect and significant higher than BBDD at sperm density and semen volume (p<0.05). We tentatively conclude that NCOA1 gene is the major gene affecting semen quality or linked to the major gene and the mutation could be used as the molecular genetic marker to select shaobo rooster for high semen quality.
Z.H. Zhao, S.F. Li, H.Y. Huang, C.M. Li and J. Zhang, 2011. Single Nucleotide Polymorphism of Nuclear Receptor Coactivator 1 Gene and Association with Semen Quality in Chicken. Agricultural Journal, 6: 269-273.