Authors : J.U. Nwawuisi, I.N. Odoh and U.M. Anaele
Abstract: In an experiment to develop yam (Dioscorea rotundata) based culture media for isolation of tuber-rot fungi of yam (Dioscorea sp.), culture media were prepared from three varieties of Dioscorea rotundata, namely Nwopoko, Obioturugo and Abi, for isolation of six causing rot fungi of yam with Potato Dextrose Agar (PDA) as standard culture medium. The six rot fungi were: Botryodiplodia theobromae, Fusarium oxysporum, Penicillium sclerotigenum, Aspergillus niger, Rhizopus nodosus and Mucor circineloides. The result of the study showed that the growth of the fungi on the yam-based culture media was equally as good as their growth on the Potato Dextrose Agar (PDA) used as standard medium, while some even gave better growth of the fungi with sporulation as high as 1.4x108 and 1.6x108 obtained, respectively on Nwopoko and Obioturugo-based agar medium.
J.U. Nwawuisi, I.N. Odoh and U.M. Anaele, 2012. Developing Culture Media from Yam Species (Dioscoreae rotundata): Technique for Isolation of Tuber-Rot Fungi of Yam. Agricultural Journal, 7: 260-263.