Authors : Jun Wang, Rui Wang and Xiao-Ye Yang
Abstract: For oral application biocontrol of animal parasitic nematode in ruminants, ion beam implantation nematode-trapping fungi must has the capacity to survive the passage through the digestive tract and be efficient in reducing infective larvae of nematodes in the faeces. Ion beam induced mutation the spores of Arthrobotrys oligospora of nematode-trapping. The mutant of the genetic stability is breeded. The fungi were cultured in bottles with corn kernels as a growth media and spores of different doses was respectively administered orally to each group of sheep naturally infected with gastrointestinal nematodes. The control group did not receive fungi. The faeces of these experimental animals were colleted and fecal cultivations carried out. Tested the fungal germination, growth, reproduction and predation livestock parasitic nematode larvae in laboratory. Test the efficacy of a Arthrobotrys oligospora N mutant in nematode-trapping larvae after passage through the digestive tract of sheep. In this research, these capacities were evaluated. The results indicated that ion beam implantation nematode-trapping fungi is a positive mutation. Mutant spores through the digestive tract of sheep can kill livestock parasite nematode larvae in vitro. These results indicate the potential of Arthrobotrys oligospora N mutant as a biological control agent for sheep nematodes. This study showed that such biotechnology can be explored for improving the effectiveness of the use of fungal infections to control livestock parasitic nematodes. This research represents the first application of nematode-trapping fungi in eukaryotic microorganisms.
Jun Wang, Rui Wang and Xiao-Ye Yang, 2013. Test the Efficacy of a Arthrobotrys oligospora N Mutant in Nematode-Trapping Larvae after Passage through the DigestiveTract of Sheep. Agricultural Journal, 8: 13-16.