Authors : Ikuo Inoue, Sanae Shibata and Tsuneo Fukata
Abstract: To compare Escherichia coli (E. coli)-DNA profile isolated from the uterus and feces of bitches suffering from pyometra, Plsed-Feld Gel Eectrophoresis (PFGE) was used. E. coli from seven bitches suffering from pyometra was isolated as 8 strains from the uterus and 15 from feces, respectively. In three out of seven bitches same DNA-profiles of E. coli colony types from the uteres and feces were shown by PFGE. It was first demonstrated by PFGE that E. coli strains isolated from the uterus and feces of bitches suffering from pyometra had the same DNA-profile. Therefore, the pathogen of pyomotra has reconfirmed as E. coli from feces by PFGE with higher homogeneity.
Ikuo Inoue, Sanae Shibata and Tsuneo Fukata, 2013. Comparison by PFGE of Escherichia coli from the Uterus and Feces of Bitches with Pyometra. Agricultural Journal, 8: 75-77.