Abstract: Lobesia botrana (Denis and Schiffermuller) grapevine moth is the most harmful pest in the fields of grape cultivation in Turkey. The present study compared the parasitoids of Lobesia botrana obtained from control area and the area where mating disruption method is applied. Pimpla spuria, Exochus erythronotus and Mesostenus transfga were the species among parasitoid species that were found at highest rate in the pupae collected in mating disruption area and the control area. In 2005, the parasitism rates were found 3.7% in Pimpla spuria, 1.3% in Exochus erythronotus and below 1% in Mesostenus transfga in mating disruption area and 1.7% in Pimpla spuria, 1% in Exochus erythronotus and below 1% in Mesostenus transfga in control area. In 2006, the parasitism rates were found 8.3% in Pimpla spuria, 3% in Exochus erythronotus and 1.5% in Mesostenus transfga in mating disruption area and 1.6% in Pimpla spuria, 1% in Exochus erythronotus and 1% in Mesostenus transfga in control area. In mating disruption vineyards and control area, especially L. botrana among the parasitoids caught with CDC Backpack insect aspirator was determined to be intensely caught in the 1st and 2nd generations. In these areas where intense disinfection is applied as a result of using bacterial preparation Bacillus thuringiensis ssp. kurstaki instead of chemical pesticide for 2 years, a remarkable increase was observed in the number of the parasitoids, especially in Pimpla spuria parasitoid species, obtained from the mating disruption vineyard in 2006.