Authors : Syamsudin , Soesanto Tjokrosanto , Subagus Wahyuono , Darmono and Mustofa
Abstract: In vitro and in vivo antiplasmodial activities of stem barks extracts of Garcinia parvifolia Miq, medicinal plant traditionally used to treat malaria in Indonesia, have been evaluated. The IC50 value of extracts was determined. Extracts of this plant (n-hexane, ethylacetate and n-buthanol extracts) were tested in vitro for their antiplasmodial activity on 2 strains of Plasmodium falciparum, FCR-3 (chloroquine-resistant strain) and 3D7 (chloroquine-sensitive strain) using a visual method. Cytotoxicity of these extracts were performed on HeLa cells in vitro using MTT method. The in vivo antiplasmodial testing used a standard 4-days test on P. berghei infected mice and acute toxicity testing on mice were also conducted. The IC50 values of the plant extracts were in the range of 4,83-40,10 �g mL 1. Significant in vitro antiplasmodial activity and higher selective toxicity were observed by n-hexane extract (IC50 = 4,11 µg mL 1 and C/A = 48,23). The n-hexane extract was the most active in vivo against P. berghei (ED50 = 19,95 mg kg 1BW) and least toxic in mice (LD50= 1060,47 mg kBW 1). These results suggest that n-hexane extract is promising extract for further investigation for new antimalarial agents.
Syamsudin , Soesanto Tjokrosanto , Subagus Wahyuono , Darmono and Mustofa , 2007. In vitro and in vivo Antiplasmodial Activities of Stem Bark Extracts of Garcinia parvifolia Miq (Guttiferae) . International Journal of Tropical Medicine, 2: 41-44.