Authors : A.M. Mat Jais , Z.A. Zakaria , A. Luo and Y.X. Song
Abstract: The present study was carried out to screen the in vitro antifungal activity of various extracts of Channa striatus (Haruan) against 13 filamentous fungus and 2 non-filamentous fungus (yeast) obtained from the China General Microbiological Culture Center (CGMCC) using the filter paper, Oxford cylinder and inoculation techniques. Both extracts 1 and 2 were prepared in distilled water under pressured cooking, but extract 2 was added with special blend of soup spices. The mucus extract was collected in distilled water as extract 3, after 1 h hypothermal shocked being introduce at -20�C to the fish. In addition, extracts 4, 5, 6 and 7 were obtained by soaking the fillet separately in methanol, ethanol, chloroform and chloroform: methanol (2:1; w v 1). Additional experiments on the fish fillet extracts� antibacterial activity against various strains of Staphylococcus aureus were also carried out using the single concentration microdilution method. The results obtained demonstrated that only extract 5 (ethanol) inhibited the growth of Neurospora crassa 3.1601, Aleurisma keratinophilum 3.3544 and Cordyceps militaris 3.4655 after 24, 46 and 64 h of treatment. The same extract also inhibited the growth of Botrytis pyramidalis 3.1930 and Paecilomyces fumosa-roseus 3.3727, but only for the first 24 and 46 h. All extracts did not show any signs of growth inhibitory effect when tested against the S. aureus strains. This is very important for although the inhibition was not strong enough to kill some of the affected fungus, the partial inhibition by an animal (fish) based extract will be of better use for human consumption to avoid unnecessary repercussion.
A.M. Mat Jais , Z.A. Zakaria , A. Luo and Y.X. Song , 2008. Antifungal Activity of Channa striatus (Haruan) Crude Extracts. International Journal of Tropical Medicine, 3: 43-48.