Authors : Asmaa M. El-kady, Osama H. Abdella, Amal A. Hassan, Yasser Mokhtar and Mohammed H. Hassan
Abstract: Schistosomiasis is one of the most devastating neglected tropical diseases. Several serological methods have been developed recent decades to diagnosis Schistosoma infections. The present study aimed to evaluate the sensitivity and specificity of ELISA and IHA in the diagnosis of both acute and chronic S. haematobium infections. The present study was conducted on 120 patients (100 were microscopically diagnosed as urinary schistosomiasis and 20 healthy controls. Serum samples from each patient were examined using ELISA and IHA. ELISA with S. mansoni soluble egg antigen was used to detect anti-Schistosoma IgG (SEA/ELISA) and IHA was performed using Worm Antigen (WA/IHA). With regard to ELISA, the sensitivity of the SEA/ELISA is 97%. Using IHA, the sensitivity of the WA/IHA with a cutoff of 1:80 (WA/IHA80) is 86%. After the combined use of ELISA and IHA, the sensitivity of the combined use of SEA/ELISA and WA/IHA80 is 100%. Analysis of the negative control cases using IHA showed that the specificity of the test was 85%. Using ELISA, the specificity of the test was 95%. Therefore, the specificity of the combined use of WA/IHA80 and SEA/ELISA is 95%. Our findings suggest that WA/IHA and SEA/ELISA are both sensitive and specific serological tests that are easy to use for the diagnosis of schistosomiasis. The combined use of these two tests enabled the serological diagnosis of schistosomiasis to be achieved with very high degrees of sensitivity and specificity.
Asmaa M. El-kady, Osama H. Abdella, Amal A. Hassan, Yasser Mokhtar and Mohammed H. Hassan, 2018. Comparative Validity of ELISA and Indirect Haemagglutination in Diagnosing Schistosoma haematobium Infection: An Egyptian Study. International Journal of Tropical Medicine, 13: 39-42.