Authors : G. Dir? , E. Lima , M. L. Gomes , M. Castro Faria , R. L. Jales and M. Bernardo-Filho
Abstract: The use of natural products as medicines has been growing in the entire world. There are concerns that these products may contain potentially toxic ingredients and contaminants such as heavy metals. The labeling of blood constituents with technetium-99m has been influenced by the presence of natural extracts. We evaluated the influence of a chayotte (Sechium edule) extract (100%vv macerated) on the labeling of blood elements with 99mTc. The animals were treated with the extract during 15 days. Samples of blood were incubated with stannous chloride and with 99mTc. Plasma and blood cells were isolated and also precipitated with trichloroacetic acid and soluble and insoluble fractions obtained. Samples were carried out with specific blood biochemistry kits. We analyzed the influence of chayotte in the survival of the strain of Escherichia coli AB1157 submitted to reactive oxygen species induced by stannous chloride. There was a decrease in the radioactivity in the insoluble fractions of plasma and a reduction of the lethal effect induced by stannous chloride on the survival of the E.coli culture in the presence of chayotte. The results indicated a decrease in the level of glucose and globulin The effect of the extract could be explained by its metabolic transformation inducing the generation of oxidant metabolites. The culture of bacteria when was treated with stannous chloride and chayotte simultaneously, the extract could be reacting with stannous chloride ions, protecting them against the oxidation avoiding the generation of reactive oxygen species.
G. Dir? , E. Lima , M. L. Gomes , M. Castro Faria , R. L. Jales and M. Bernardo-Filho , 2003. Effects of a Chayotte (Sechium edule) Extract (Macerated) on the Labeling of Blood Constituents with Technetium-99m, on the Biochemistry of Blood of Wistar Rats and on the Action Against the Stannous Chloride Effect . Journal of Animal and Veterinary Advances, 2: 602-606.