Authors : Sara Abudafeera , Mariana Nor Shamsudin , Son Radu , Rozita Rusli and Osman Elobied
Abstract: Enterohemorrhagic Escherichia coli (EHEC) of serotype O157 causing hemorrhagic colitis is on the increase in many parts of the world, hence specific surveillance of this pathogen is essential for identifying the sources and monitoring its spread. In this study, a total of 22 imported beef isolates of E. coli O157 isolated from wet market in Seri Serdang in Malaysia and 24 imported lamb isolates of E. coli O157 isolated from Center Slaughterhouse in Al-Ain in United Arab Emirates were examined. All the isolates were investigated by randomly amplified polymorphic DNA (RAPD) analysis with one primer: Gen1-50-9 (5?-AGAAGCGATG-3?). The primer generated polymorphisms in most of the isolates of E. coli O157 tested, producing bands ranging from 0.25 to 4.0 kilobases. The RAPD profiles revealed a high level of DNA sequence diversity within E. coli O157 isolates tested.
Sara Abudafeera , Mariana Nor Shamsudin , Son Radu , Rozita Rusli and Osman Elobied , 2004. Analysis of of Escherichia coli O157 isolated from imported beef and lamb in Malaysia and United Arab Emirates using RAPD-PCR . Journal of Animal and Veterinary Advances, 3: 148-153.