Authors : S. Biswas , A. Sanyal , D. Hemadri , C. Tosh , S. K. Gupta , M. Mittal , J. K. Mohapatra , G. P. Sabarinath , R. Manoj Kumar and S. K. Bandyopadhyay
Abstract: Foot-and-Mouth Disease (FMD), the most economically important disease of cloven-hoofed animals is endemic in India. A total of twenty field isolates belonging to serotype Asia1, collected from different states in India during 1986-2002 were taken as BHK-21 cell culture adapted virus from the National Repository of FMDV at the Project Directorate on Foot-and-Mouth Disease (PD FMD). RT-PCR products of the revived viruses were confirmed by agarose gel electrophoresis and the gel-purified products were subjected to cycle sequencing in an automated sequencer. Vpg protein, which on uridylation serves as a primer during FMDV replication is found conserved across serotypes O, A and C. The sequences generated in the present work were compared with respect to other serotypes with the objective of investigating the amino acid variability of the three redundant copies of VPg proteins in a diverse group field isolates of type Asia1.
S. Biswas , A. Sanyal , D. Hemadri , C. Tosh , S. K. Gupta , M. Mittal , J. K. Mohapatra , G. P. Sabarinath , R. Manoj Kumar and S. K. Bandyopadhyay , 2004. Sequence Analysis of VPg Protein of Asia1 Field Isolates of Foot-and-Mouth Disease Virus in India . Journal of Animal and Veterinary Advances, 3: 522-526.