Abstract: The specific objectives, in the present study, were to compare the reproductive performance such as egg diameter, number of peleopod egg and relative physiological conditions of female white river crayfish in different habitats. Fecundity of adult female Procambarus acutus acutus were examined in simulated burrows and excavated pond burrow for three months. Pleopod egg numbers per female increased linearly with crayfish TL. Pleopod egg numbers varied from 106 to 556 for 81 and 127 mm Total Length (TL) female in simulated burrow and from 32 to 330 eggs for 65 mm and 125 TL female in excavated pond burrows, respectively. Similar sized female crayfish in simulated burrow oviposited significantly more eggs than the crayfish species in excavated pond burrows. The slopes and intercept of the TL regression for pleopod eggs for female were significantly different between those individuals held in simulated burrows and excavated pond burrows (p<0.05). HM content of P.a. acutus held in simulated burrow (47.1%) was less than that of the excavated pond burrows (62.2%) (p<0.05). The diameters extruded eggs for the two burrow types were similar during the study period.