Abstract: Rainbow trout (Oncorhynchus mykiss) sperm was collected without anesthesia by abdominal massage. In collected sperm, volume, motility, movement duration, density, total density and pH were recorded. Furthermore, body weight and body length were measured and correlations beetween spermatological properties and these parameters were assessed. Fertilization was carried out using the dry fertilization technique at 1x105 spz/egg ratio. The highest fertilization and hatching rates were found as 93 and 100%, respectively. There were significant positive correlations between body weight and length (r= 0.800, p<0.01), body weight and movement duration (r= 0.556, p<0.05), body length and pH (r= 0.613, p<0.05) and density and total density (r= 0.864, p<0.01). Significant negative correlations were found between body length and density (r= -0.609, p<0.05) and body length and total density (r= -0.570, p<0.05). In addition, it was observed that there was a positive relation between motility and fertilization rate (0.944, p<0.01) and also between motility and hatching rate (0.689, p<0.01). Furthermore, fertilization and hatching rates significantly correlated (r= 0.742, p<0.01) with each other.