Authors : Ikram Ullah , Samina Jalali , Sajjad A. Shami , Khalid Farooq and Muhammad Ijaz Khan
Abstract: The present study was conducted to investigate the effect of 2-mercaptoethanol (ß-ME) on Nile Ravi buffalo oocytes. Cumulus Enclosed Oocytes (CEOs) and Cumulus Denuded Oocytes (CDOs) were cultured for 24 hrs in TCM-199 supplemented with Recombinant Human Follicular Stimulating Hormone (RhFSH), 0.4% Bovine Serum Albumin (BSA), oestradiol and Gentamycin. Nili Ravi buffalo oocytes with or free from cumulus cells were exposed to 25 �M 2-ME. Meiotic maturation and degeneration of oocytes was assessed at different maturation time (0, 12, 24 h). CDOs treated with 25 �M 2-ME resumed meiosis and proportion of MII oocytes were significantly (P < 0.05) increased where as CDOs matured in control medium with out 25 �M 2-ME did not show any significant increase in MII oocytes proportion as the maturation time was extended to 24 h (p<0.05). Similarly CDOs treated with 25 �M 2-ME significantly reduced the proportion of degenerated oocytes (7.31 %) against control group in which 21.4 % CDOs degenerated. In present study it was observed that cumulus cells are involved in protection against degeneration as CEOs treated in control medium the degenerated oocytes high significantly (p<0.05) decreased to 7.40 % as compared to CDOs in which 21.4 % oocytes were found to be degenerated. In present study the proportion of MII oocytes was significantly increased to 81.81 % when CEOs were treated with 25 �M 2-ME against CDOs treated with 25 �M 2-ME after 24 hrs of culture. These results indicates that that exposure to 25 �M 2-ME promote the development of the Nili Ravi buffalo CDOs and CEOs and cumulus cells are involved in protection against degeneration of oocytes.
Ikram Ullah , Samina Jalali , Sajjad A. Shami , Khalid Farooq and Muhammad Ijaz Khan , 2006. Effect of the 2-Mercaptoethanol on Nili Ravi Buffalo Oocytes During In vitro Maturation Order of Authors. Journal of Animal and Veterinary Advances, 5: 380-385.