Authors : N.D. Seifollah , A. Aliabadi and S.T. Nejad
Abstract: Bone Morphogenic Protein (BMP) can cause molecular and cellular impulses and induce rapid bone healing process. Therefore, it is good for cases of delayed or non-union fractures as well as filling up of intervertebral gaps by osteogenic activity. The bovine long bone were collected and processed through defating, grinding, decalcification, degreasing, protein extraction, dialyzing, purification and lyophilizatioan. The extracted BMP was kept in the refrigerator. Ten adult horses of both sex were used and divided randomely into 2 equal groups. The right metetarsus was prepared and anesthesia was induced by xylazin, diazepam and ketamin Hcl and maintained with halothane 2%. All horses were handled according to Shiraz University regulation for animal rights. Under general anesthesia a window defect was made through midmetatarsal region by electric osteotom in all horses. The horses of the first group received BMP that was injected at the window site of metatarsal bone. The wound was closed, bandaged and splinted in both groups. They were monitored clinically for 12 weeks and histomorphometric evaluation were performed after 12 weeks. Clinically the horses in BMP group were using their legs slightly from 28th and completely on 42th postoperative day but the horses in control group did not use their legs even on 42th postoperative day. Histomorphometric data showed better thickness of cortical and trabecular bone in treatment group and periosteum was peresent and intact at the window site in patients of treatment group compared to control group.It can be concluded that the extracted protein was BMP and it had accelerated the bone healing process by osteogenic activities.
N.D. Seifollah , A. Aliabadi and S.T. Nejad , 2007. Effect of Bone Morphogenic Protein on Bone Healing Process in the Horse: Histomorphological Study . Journal of Animal and Veterinary Advances, 6: 1242-1246.