Authors : Khairelsid Abdalla , Tal b Mohamed , Khalifa Khalifa and Salah Al-Izzi
Abstract: This study was performed to transfer the immunity against R. equi by using sensitized spleen cells in guinea pigs. Firstly, two groups of guinea pigs were used, the first injected i.m with formalin killed antigen prepared from R. equi mixed with in complete Fruend�s adjuvant at two weeks interval. While the second was inoculated with the adjuvant plus Phosphate Buffer Saline (PBS) and kept as control. The immune response, both humoral and cellular were monitored using tube agglutination test, passive haemagglutination test, skin test, E-rosette and migration inhibitory factor in treated groups. Injection of animals induced both types of immune response, the treated and control groups considered as donors for sensitized and non-sensitized spleen cells, respectively. Secondly, three groups were used as recipients, the first one injected i.p with 2 mL of 5X108 cell mL-1 sensitized spleen cells, the second group similarly injected with non-sensitized cells and the third one injected with PBS only then kept as control. The immune response was monitored in the recipients which revealed specific R. equi antibodies and developed Delayed Type Hypersensitivity (DTH). Statistical analysis was done using one way analysis of variance and F-test which revealed a high significant differences among treated and control groups (p<0.05). The results suggest that sensitized spleen cells of guinea pigs has an effect on other recipients and concluded that transfer of sensitized cells were effective in R.equi specific immune response in comparison to the control.
Khairelsid Abdalla , Tal b Mohamed , Khalifa Khalifa and Salah Al-Izzi , 2007. Transfer of Rhodoccocus equi Immunity in Guinea Pigs by Mean of Sensitized Spleen Cells . Journal of Animal and Veterinary Advances, 6: 223-233.