Authors : Siriphan Ponsen , Nual-Anong Narkkong and Warapol Angwanich
Abstract: The objective of this study was to examine the erythrocytes, leukocytes and thrombocytes of the sand lizard (Leiolepsis belliana rubritaeniata Mertens 1961) by light and electron (SEM and TEM) microscopy. Smears were prepared from blood from the heart of ten healthy adult sand lizards (five males and five females). Electron microscopy was also performed on all samples. The results revealed the following information: light microscopy finding; erythrocyte, lymphocyte, monocyte, heterophil, basophil, eosinophil and thrombocyte presented district morphology. Erythrocytes, mononuclear cells, granulocytes and thrombocytes of the sand lizard were nearly similar to those of chickens, snakes, tortoises, turtles and other lizards. SEM finding, the lymphocyte showed tiny round cells. The monocyte was larger than the lymphocyte and had a round cell shape and rough membrane. The heterophil was a round or elongated cell with a rough membrane. The basophil was tiny and round with a rough membrane surface. The Eosinophil was a round cell with many spherical spines protruded from their membrane surface. The thrombocyte was a tiny cell with an irregular membrane. TEM finding, the nucleus of the lymphocyte was large and round with heterochromatin. The nucleus of the monocyte was mononuclear, kidney shaped with heterochromatin. The nucleus of the heterophil was lobuled with heterochromatin, dense cytoplasm and contained spindle, drum-bell or long shape granules. The nucleus of the basophil was lobuled with heterochromatin and the cytoplasm contained large amount of strip granules. The nucleus of the eosinophil was round and had a dense cytoplasm and contained large bowling pin and round-shape liked granules. The nucleus of thrombocyte was dense with clear cytoplasm and no organelle appearance.
Siriphan Ponsen , Nual-Anong Narkkong and Warapol Angwanich , 2007. Morphological and Ultrastructural Observations on the Blood Cells of Sand Lizards (Leiolepis belliana Rubritaeniata) Mertens 1961 . Journal of Animal and Veterinary Advances, 6: 522-527.