Authors : N. Hadjipour , S.M. Naghib and M. Davanian
Abstract: Gentamicin (GM) is widely used as a bactericidal agent for treatment of severe gram negative infections. However, the clinical use of gentamicin is limited due to its known nephrotoxic effects. Previous studies were done on other antioxidant vitamins and in this study we directly compare the effects of Vitamins A and C on reduction of gentamycin nephrotoxicity on rat kidney. Thirty adult male Sprague-Dawley rats were randomly assigned to 5 groups: group 1(control) was injected with strile normal saline; group 2 (Gm 5-8 mg kg-1 IM); group 3(GM +VitA); group 4(GM + VitC); group 5(GM + VitA + VitC). The injection courses for all groups were 14 days. Then histopathologic slides were provided from these kidneys. In slides observations by light microscope, 4 levels of necrosis were defined (the level-making method will be mentioned in full text): 1 = light necrosis, 2 = medium necrosis, 3 = severe necrosis and 4 = extra severe necrosis. The 6 rats in second group (injected by Gentamycin) showed levels 3,4,4,4,3,4 of necrosis in kidney (average:3/66). Co-injecting of Vit. A with Gentamycin had a trivial effect on nephrotoxicity reduction, so that the necrosis levels in the rats of this group were 4,3,3,3,3,4 (average:3/3). The rats injected by GM + VIT.C showed a remarkable effect on the reduction of Gentamycin nephrotoxicity by causing 1,2,2,1,1,1 levels of necrosis (average: 1/33). The last group (co-injecting of GM, VIT.A and VIT.C) showed a average of 2/5 in necrosis level (levels of 3,3,2,3,2,2). So by statistical analysis it can be concluded that combination of Vit. C with Gentamycin administration can be the drug of choice in infection treatments to reduce Gentamycin nephrotoxicity.
N. Hadjipour , S.M. Naghib and M. Davanian , 2008. Histopathological Study of Vitamins A and C Effects on the Reduction of Gentamycin Nephrotoxicity in Rats. Journal of Animal and Veterinary Advances, 7: 1038-1041.