Authors : R. Asadpour , F. Rezazadeh and H. Hamali
Abstract: The objective of this study, was to evaluate testosterone profiles of serum in buffalo bulls and to examine its correlation with semen characteristics. Semen of 10 buffalo bulls were collected by a bovine artificial vagina. Semen characteristics such as (motility, morphology, viability and concentration) were recorded. A part of the semen sample (1 mL) was diluted by tris-egg yolk-glycerol extender, packed in French straws and was frozen in liquid nitrogen. The straws were later thawed and semen characteristics were compared with those of the fresh semen. The blood samples were collected in vacutainers without anticoagulant. After 1 h at room temperature, the samples were centrifuged at 2000 rpm for 20 min and then the sera were collected, immediately frozen and thereafter kept at -20�C until the hormone assay, performed by RIA. The results showed the overall Mean�S.E. blood testosterone concentration was 1.3�0.9 ng mL 1. Blood serum testosterone was correlated with progressive motility and viability of the fresh and frozen-thawed semen. Progressive motility, viability and abnormal sperm morphology of frozen-thawed semen were highly correlated with mentioned above parameters in the fresh semen. Testosterone serum concentrations in buffalo bulls are similar to those reported in other animal species and have some correlations with semen characteristics before and after freezing.
R. Asadpour , F. Rezazadeh and H. Hamali , 2008. Blood Testosterone Level in Iranian Buffalo Bulls and its Relation with Semen Freezability. Journal of Animal and Veterinary Advances, 7: 1559-1562.