Authors : Marek Kmiec , Daniel Polasik , Filip Napierala and Iwona Polasik
Abstract: The study covered a herd of 300 polish large white sows kept in one pigsty in the West-Pomeranian Region in Poland. The polymorphism of a selected 506-bp GH gene fragment was detected by the PCR-RFLP method using specific primer sequences and endonuclease HaeII and MspI. In the studied herd of sows, there were found to be three GH/HaeII genotypes (h-h--4.7%, h+h--54% and h+h+-41.3%) and three GH/MspI genotypes (m-m- 30%, m+m--23.3% and m+m+-46.7%), each of them determined by two alleles. The GH/HaeII allele frequencies were 31.7% for allele h- and 68.3% for allele h+, whereas the GH/MspI allele frequencies were 41.7% for allele m- and 58.3% for allele m+. The genetic equilibrium in the herd under study was found to be disrupted as the number of individuals observed in the GH/HaeII and GH/MspI genotype groups was significantly different from their expected number calculated according to the Hardy-Weinberg law. The study proved the existence of GH/HaeII and GH/MspI polymorphisms in a selected GH gene sequence in Polish Large White sows and revealed an association between the GH/HaeII and GH/MspI genotypes and reproductive traits of the sows.
Marek Kmiec , Daniel Polasik , Filip Napierala and Iwona Polasik , 2008. GH/HaeII and GH/MspI Restriction Polymorphism in a Herd of Polish Large White Sows. Journal of Animal and Veterinary Advances, 7: 165-169.