Authors : P.M. Hernandez-Mendoza , K.Y. Coronado-Manzanares , A.M. Nevarez-Garza , L.E. Rodriguez-Tovar , G. Hernandez-Vidal , J.A. Salinas-Melendez and R. Ramirez-Romero
Abstract: Herein, we explored the number of infiltrating mast cells in the skin of diabetic rabbits during the acute phase of the inflammatory response induced by an active Arthus reaction. New Zealand Rabbits were divided in 5 groups (n = 5/group). One group served as control while the others were subjected to the reaction. Two Arthus groups (groups 1 and 2) received alloxan to induce diabetes 75 days before the hyperimmunization protocol. Diabetes was confirmed by blood glucose tests 48 h after alloxan injection and at the time of euthanasia. An agar immunodifussion test showed that diabetics and non-diabetics (groups 3 and 4) had enough serum antibodies by the time of euthanasia. Rabbits were euthanized at 8 h (groups 1 and 3) and 24 h (groups 2 and 4). Controls (group 5, non-Arthus, non-diabetes) received saline and were killed at 24 h. Skin samples were fixed in Carnoy`s solution and mast cells were identified employing a low pH toluidine blue stain. Mast cells were quantified per square millimetre and then, those degranulated cells were registered to obtain absolute values. An ANOVA test employing a completely random design and subsequently, a Tukey`s w test were carried out. Arthus reaction influenced markedly mast cell infiltration. Although, diabetic animals had fewer mast cells than non-diabetics (p<0.05), numbers of degranulated cells were similar among them (p>0.05). Diabetes markedly decreases the infiltration of mast cells during the active Arthus reaction in the skin. However, their degranulation status is comparable with non-diabetics.
P.M. Hernandez-Mendoza , K.Y. Coronado-Manzanares , A.M. Nevarez-Garza , L.E. Rodriguez-Tovar , G. Hernandez-Vidal , J.A. Salinas-Melendez and R. Ramirez-Romero , 2008. Diabetes Decreases Mast Cell Infiltration During the Acute Phase of the Active Arthus Reaction. Journal of Animal and Veterinary Advances, 7: 620-624.