Authors : Mohammad Mehdi Kasiriyan , Hasan Hafezeyan , Hadi Sayahzadeh , Reza Jamshidi , Saied Reza Asghari , Gholam Hossein Irajeyan and Hamzeh Buesagh
Abstract: Phenotypic evaluation and culling of candidate animals for traits by applying traditional animal breeding are usually costly tasks, which require considerable time to be carried out. Molecular genetic as an alternative method, enables animal breeders to select eligible animals for the desirable trait(s) at their earlier ages. Selection based upon markers could result in increasing accuracy as well as selection response of animals. This study was carried out to evaluate the genetic polymorphisms in BMP15 and FecB in Iranian Sangsari sheep breed. Blood samples were taken from 150 Sangsari sheep (140 ewes, 10 rams) from sheep in Damghan genetic modification center and the genomic DNA was extracted using salting out method. After the extraction and quantitative and qualitative tests (80% spectrophotometer and gel agarose 8%) the required amounts for each Polymerase Chain Reaction (PCR) were specified. Using 2 pairs of specific primers, 2 DNA fragments were amplified from exon 2 of BMP15 (141 bp) and FecB (190 bp) genes. The resulted PCR products were digested using HinfI and AvaÎ restriction enzymes for BMP15 and FecB genes, respectively. Genotypes of each individual were detected by agarose gel electrophoresis. Restriction digested of PCR products for BMP15 locus with Hinf I enzyme showed a C to T transition. BMP15 and FecB luci were not polymorphic. Further studied required to evaluate the relationship of different genotypes with litter size and ovulation rate.
Mohammad Mehdi Kasiriyan , Hasan Hafezeyan , Hadi Sayahzadeh , Reza Jamshidi , Saied Reza Asghari , Gholam Hossein Irajeyan and Hamzeh Buesagh , 2009. Genetic Polymorphism FecB and BMP15 Genes and Its Association with Litter Size in Sangsari Sheep Breed of Iran. Journal of Animal and Veterinary Advances, 8: 1025-1031.