Authors : K. Hamasu , K. Shigemi , Y. Tsuneyoshi , H. Sato , D.M. Denbow and M. Furuse
Abstract: The aim of the present study was to determine whether the sedative effects of L-proline are associated with the modulation of cholinergic neurotransmission. We investigated the effect of co-injection of L-proline with scopolamine, a Muscarinic Acetylcholine Receptor (M-AChR) antagonist, on behavior of neonatal chicks under isolation-induced stress. Intracerebroventricular (i.c.v.) injection of L-proline reduced spontaneous activity and the number of distress vocalizations, while co-injected scopolamine did not attenuate this effect implying that the M-AChR was not involved in the sedative effects induced by L-proline. In addition, the effect of L-proline on acetylcholineesterase activity in the telencephalon and diencephalon of chicks was investigated. No significant changes in acetylcholineesterase activity were observed in either the telencephalon or diencephalon. These results indicate that the sedative effects induced by L-proline are not mediated by the cholinergic system.
K. Hamasu , K. Shigemi , Y. Tsuneyoshi , H. Sato , D.M. Denbow and M. Furuse , 2009. Cholinergic Neurotransmission is not Involved in Sedation Induced by L-Proline in Neonatal Chicks. Journal of Animal and Veterinary Advances, 8: 1207-1211.