Abstract: The objective of the study was to determine body weight loss during transportation of chickens into the Slaughtering house. For this reason, after 42-days of feeding, the male and female broilers were separated into three weight groups such as <2200 g (group A, light weight), 2200-2500 g (group B, middle weight) and >2500 g (group C, heavy weight). Also, female birds were separated into three groups according to live body weights such as <2000 g (group A, light weight), 2000-2200 g (group B, middle weight) and >2200 g (group C heavy weight). Three crates (7/crate) were used for each sex and for each live weight group. Transportation periods were stated as 1-3 h. Live weights of the all chickens were recorded prior to end of the transport period. Mortality and carcass performances also were determined. Weight lost of the male chickens from group A was determined as 46.81, 64.43 and 106.15 g for 1-3 h transport periods, respectively and the differences among three periods were found to be significant. Weight lost of male chickens for group B was determined as 43.10, 94.05 and 120.42 g for 1-3 h transport periods, respectively and the differences among three periods were found to be significant. Weight lost for group C chickens were determined as 92.57, 138.87 and 140.94 g for 1-3 h periods, respectively. For the group A, weight lost of female chicks were recorded as 33.67, 50.90 and 73.33 g for 1-3 h periods, respectively. Main lost was found for 3 h period (compare with two other periods), which was significant. Body weight lost for group B chicks were determined as 37.76, 60.67 and 87.37 g for the same periods, respectively. As conclusion, transportation of the chickens is a great stress factor and has adverse effects on the live weight of the birds.