Abstract: This experimental was conducted to determine the effect of the Residual Oil (RO) on cholesterol and Malondialdehyede (MDA) as an indicator of lipid oxidation in muscles (breast and thigh) of male broiler chickens. A total of 108 Ross-308 strains were randomly divided into 3 experimental treatments with 3 replicates (12 chicks per pen) and arranged in a Completely Randomized Design (CRD). Experimental diets consisted of: Basal diet with 0% RO; basal diet with 2.5% RO and basal diet with 5% RO. These diets were isonitrogenous and isoenergetic. The feeding continued up to the age of 42 days. Two male birds selected with each pen and slaughtered. Data was analyzed with one way ANOVA and means compared with Duncan test. The results indicated that levels of 5 and 2.5% RO (T₃ and T₂, respectively) caused significant increases in breast and thigh muscles MDA and cholesterol contents as compared with control group (p<0.05). These results suggest that usage RO in the broilers diet may raise the susceptibility of tissues to free radical oxidative damage.