Authors : Sebnem Pamuk and Sadi Akgun
Abstract: The aim of this present study is to detect thermophilic Campylobacter species in chicken carcasses sold unpackaged in Afyonkarahisar and to confirm C. jejuni isolates by using a PCR test based on the ceuE gene. A total of 210 chicken carcasses were collected from various markets from May till the end of October in 2004. Thermophilic Campylobacter sp. was isolated from 141 (67%) of 210 chicken carcass samples by classical culture method. The identification of 419 thermophilic Campylobacter sp. isolates obtained from chicken carcass samples were; 57.04% (239) C. jejuni, 40.57% (170) C. coli and 2.39% (10) C. lari. PCR method was applied to 101 of 239 C. jejuni isolates obtained from the chicken carcasses (n = 101). According to it 62 (61.38%) of 101 isolates were confirmed by PCR. About 28 (45.16%) of 62 isolates were confirmed to be C. jejuni were detected to be resistant to nalidixic acid.
Sebnem Pamuk and Sadi Akgun, 2009. Detection of Thermophilic Campylobacter sp. in Unpacked Broiler Carcasses in Retail Markets of Afyonkarahisar and Confirmation C. jejuni Isolates Using PCR. Journal of Animal and Veterinary Advances, 8: 2063-2068.