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Journal of Animal and Veterinary Advances
Year: 2010 | Volume: 9 | Issue: 11 | Page No.: 1639-1645
DOI: 10.3923/javaa.2010.1639.1645  
Characteristics of Proliferation and Differentiation-Dependent C/EBP, PPARγ and Leptin Gene Expression Patterns Intrinsic to Bovine Intramuscular, Perirenal and Subcutaneous Preadipocytes
Makiko Yamamoto , Yukio Taniguchi , Koichiro Kano and Takahisa Yamada
 
Abstract: Adipose depotsite-specific variability in physiological characteristics of preadipocytes could be in part, intrinsic to the cells and become evident through in vitro proliferation and differentiation-dependent gene expression patterns in preadipocyte in the cell culture. This study was designed to characterize proliferation and differentiation-dependent gene expression patterns intrinsic to intramuscular, perirenal and subcutaneous preadipocytes. About 3 type preadipocytes are prepared using the ceiling culture method from bovine intramuscular, perirenal and are demonstrated subcutaneous adipose depots of a single Japanese Black steer. Adipose depot site-specific proliferation and differentiation-dependent gene expression patterns intrinsic to preadipocyte by comparing the mRNA levels of CCAAT enhancer binding protein C/EBPα, C/EBPβ, C/EBPδ, Peroxisome Proliferator-Activated Receptor γ (PPARγ) and leptin before and after the induction of differentiation among culture of the 3 type preadipocytes. These results may suggest the existence of the site-specific preadipocyte-intrinsic proliferation and differentiation program.
 
How to cite this article:
Makiko Yamamoto, Yukio Taniguchi, Koichiro Kano and Takahisa Yamada, 2010. Characteristics of Proliferation and Differentiation-Dependent C/EBP, PPARγ and Leptin Gene Expression Patterns Intrinsic to Bovine Intramuscular, Perirenal and Subcutaneous Preadipocytes. Journal of Animal and Veterinary Advances, 9: 1639-1645.
DOI: 10.3923/javaa.2010.1639.1645
URL: http://medwelljournals.com/abstract/?doi=javaa.2010.1639.1645