Authors : Li-Ying Geng, Chuan-Sheng Zhang and Yun-Liang Jiang
Abstract: Bone Morphogenetic Protein 11(BMP11) acting as a regulator of anterior/posterior patterning in the axial skeleton is known to play a key role in murine bone morphogenesis. In pigs, the number of thoracic vertebra and lumbar vertebra is an economically important trait affecting body size and BMP11 is assumed to be involved in this process. With primers designed according to the homologous sequence of human and murine BMP11, the porcine BMP11 exon3 was first obtained by PCR. The intron2 was subsequently amplified based on primers designed from exon3 and a degenerate forward primer. The exon 2 and partial exon 1 were cloned by use of the RT-PCR and 5’-RACE approach. The cDNA sequences and the protein sequences share high sequence identity with their mammalian counterparts being 95.7, 94.0, 93.5% with human, mouse and rat cDNA sequences and 99.4% for the amino acids whereas the corresponding value is only 74.8 and 81.2% with zebra fish.
Li-Ying Geng, Chuan-Sheng Zhang and Yun-Liang Jiang, 2010. Molecular Cloning and Expression Analysis of Porcine Bone Morphogenetic Protein 11 (BMP11) Gene. Journal of Animal and Veterinary Advances, 9: 2986-2989.