Authors : Ling Yang, Jian-Guo Li, Bin Wang and Le-Ying Zhang
Abstract: Interferon-tau is generally regarded as the primary signal for maternal recognition in ruminant animals. Expression of interferon-stimulated gene 15 (ISG15) is up-regulated in bovine endometrium during early pregnancy. The experimental materials were from our primary experiment. Tissue explants of endometria from day 18 of pregnant and non-pregnant cows were cultured in vitro for 60 days and then the expression of ISG15 in these explants was detected by western blot. The results indicated that ISG15 and conjugated proteins were still expressed at a high level in endometrial explants from pregnant cows after cultured in vitro for 60 days and there was no expression in endometrial explants from non-pregnant cows after cultured in vitro for 60 days. In conclusion, expression of ISG15 in endometrial explants from early pregnant cows maintained in vitro for a long time.
Ling Yang, Jian-Guo Li, Bin Wang and Le-Ying Zhang, 2010. Expression of Interferon-Stimulated Gene 15 in Endometrial Explants from Early Pregnant Cows in vitro for 60 Days. Journal of Animal and Veterinary Advances, 9: 3016-3020.