Authors : N.S. Al-Hyali, M.A. Aljawady and M.A. Mohammad-Fakhri
Abstract: The effect of physical (heat, pH level, precipitation and dialysis) and chemical (enzymes) evaluation of sarcotoxin toxicity were investigated. After the preparation of the macrosarcocysts extract, biochemical analysis using chromatography was carried out to characterize and define the sarcotoxin of Sarcocystis gigantean. The study involved 51 albino rats, which were divided into 6 groups. Result showed that inoculation of a 1 mg mL-1 concentration of protein (sarcotoxin) of Macrosarcocysts Extract (ME) Intraperitoneally (IP) into the rats was lethal. The findings also revealed that sarcotoxin was thermolabile at 70°C for 30 min. Protease and trypsin caused inactivation, while papain had no toxic effect. Sarcotoxin was found to be non-dialyzable, but perceptible. There was also no effect of a pH level 6-9, while acidity of 4-5 altered the toxicity however, pH of 10 only had a partial toxic effect.
N.S. Al-Hyali, M.A. Aljawady and M.A. Mohammad-Fakhri, 2010. The Influence of Some Physio-Chemical Properties of Sarcotoxin in Rats. Journal of Animal and Veterinary Advances, 9: 302-305.