Abstract: This study was conducted in Province of South-East of Turkey (Islahiye District, Gaziantep) in 2005 and 2006 to test efficacy of mating disruption technique with Isonet-L dispensers against the european grapevine moth (Lobesia botrana Den. et. Schiff.) in a vineyard of the variety Antep Karasi of about 1.8 ha. Control plots (about 2.1 ha of the variety Antep Karasi) was ptotected by insecticides. The infestation rates were found as 6, 3 and 5% for the 1st, 2nd and 3rd generations, respectively in 2005. Bacillus thuringiensis sp. kurstaki were used two times against it’s 1st and 3rd generation in the plots with mating disruption treatment. The corresponding levels of infestation rates of 20, 18 and 3% for the 1st, 2nd and 3rd generations, respectively in the control area, where chemical insecticides were used 5 times. In 2006, in the plots with mating disruption treatment, infestation rates were found as 5, 2 and 3% for the 1st, 2nd and 3rd generations respectively. Bacillus thuringiensis sp. kurstaki were used once against 1st generation in plots with mating distruption treatment. Chemical insecticides were used 7 times in control plots in 2006 and infestation rates were found as 3, 0 and 0% per 100 cluster for the 1st, 2nd and 3rd generations respectively. In conclusion, this study suggests that mating disruption technique can be used against L. botrana on a large scale, unless it should be supported by a biological insecticide as Bacillus thuringiensis sp. kurstaki as infestation rate exceeds the economical threshold levels.