Authors : Fang Xing-Tang, Xu Hai-Xia, Chen Hong, Zhang Chun-Lei, Hu Xiu-Cai, Gao Xue-Yuan, Gu Chuan-Wen, Yue Wang-Ping and Lan Xian-Yong
Abstract: Bone Morphogenetic Protein 4 (BMP4) plays a crucial role in development and productivity of mammalian. The aim of the present study was to identify and characterize polymorphisms within the coding region, the intron region and the 3'flanking region of the goat BMP4 gene in three different breeds. Three DNA fragments were amplified by Polymerase Chain Reaction (PCR) and then used for polymorphism identification by Single Stranded Conformation Polymorphism (SSCP). The fragments showing different SSCP patterns were sequenced. As a result, two new SNPs (EU104684:g.1986A>G, 2203G>A) were identified in the intron region and a short sequence with more than ten continuous and repeated CA dinucleotide were found in the 3'flanking region near the termination site of coding region and the length of repeated sequence dinucleotides CA was different in samples. Clonal sequencing analysis indicated that it was a microsatellite of dinucleotide-repeated sequence. The lengths of repeated sequence dinucleotide CA were 24, 17, 14, respectively. The PIC (Polymorphism Information Content) values in three populations were 0.3656, 0.5792, 0.3103 for Xuhuai White goat, Boer goat and Haimen goat, respectively, which indicated this goat microsatellite locus had rich polymorphism.
Fang Xing-Tang, Xu Hai-Xia, Chen Hong, Zhang Chun-Lei, Hu Xiu-Cai, Gao Xue-Yuan, Gu Chuan-Wen, Yue Wang-Ping and Lan Xian-Yong, 2010. Polymorphisms of Bone Morphogenetic Protein 4 (BMP4) Gene in Goats. Journal of Animal and Veterinary Advances, 9: 907-912.