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Journal of Animal and Veterinary Advances
Year: 2011 | Volume: 10 | Issue: 14 | Page No.: 1863-1866
DOI: 10.3923/javaa.2011.1863.1866  
The Use of a Mixture of esat-6 and cfp-10 Protein as Antigens in ELISA for the Diagnosis of Bovine Tuberculosis in South China
X.D. Hu , K. Jia , G.H. Zhang , Z.X. Lin , H.Y. Xia , D.W. Zhang , X.B. Zeng , H.S. Qiao , H. Zhang and S.J. Li
 
Abstract: Several Enzyme-Linked Immunosorbent Assay (ELISA) methods exist to diagnose Bovine Tuberculosis (BTB). In this study, an ELISA method was established with a mixture of esat-6 and cfp-10 as the diagnostic antigens. ELISA tests on 160 bovine serum samples (80 from cattle with BTB; 80 from healthy cattle) demonstrated that the sensitivity was 76.3% (61/80) and the specificity was 97.5% (78/80). A further screening test using ELISA and PPD was conducted with 335 serum samples collected at random from eight dairy herds (5 problematic herds and 3 BTB-negative herds) in Guangdong, China. In the problematic herds, the positive ELISA rate was 7.9% (16/202) for anti-esat-6 and cfp-10 antibodies and 5.4% (11/202) in the PPD. This study demonstrated that ELISA with the use of the esat-6 and cfp-10 mixture of antigens is simple and sensitive and can be used to analyze large numbers of samples for the serodiagnosis of BTB.
 
How to cite this article:
X.D. Hu, K. Jia, G.H. Zhang, Z.X. Lin, H.Y. Xia, D.W. Zhang, X.B. Zeng, H.S. Qiao, H. Zhang and S.J. Li, 2011. The Use of a Mixture of esat-6 and cfp-10 Protein as Antigens in ELISA for the Diagnosis of Bovine Tuberculosis in South China. Journal of Animal and Veterinary Advances, 10: 1863-1866.
DOI: 10.3923/javaa.2011.1863.1866
URL: http://medwelljournals.com/abstract/?doi=javaa.2011.1863.1866