Authors : Chuan-Sheng Zhang, Li-Ying Geng, Yang-Qing , Wen-Jin Zhu, Qiu-Yue Wang, Xiang-Zhai Zhang, Hong-Nuan Sun, Xiao-Song Li, Rui-Zao Liu and Xiao-Hui Liu
Abstract: The aim of this study was first to investigate the evolutionary conservatism of mir-1657 among in different species and then to investigated a G-A polymorphism in the seed region of mature gga-mir-1657 sequence with forced PCR-RFLP using Ava III nuclease in six chicken populations; 178 individuals from Beijing Fatty chicken (BF), Wenshangluhua chicken (WL), Jiningbairi chicken (JB), Siyuwugu chicken (WG) and Langya chicken (LY) breeds. Bioinformatics analyses indicated that gga-mir-1657 gene G>A polymorphism may alter target selection and secondary structure. The findings indicate that the rs14934924 SNP may exert profound biological effects in the formation of some special phenotype of chicken and enables functional annotation of gga-mir-1657.
Chuan-Sheng Zhang, Li-Ying Geng, Yang-Qing , Wen-Jin Zhu, Qiu-Yue Wang, Xiang-Zhai Zhang, Hong-Nuan Sun, Xiao-Song Li, Rui-Zao Liu and Xiao-Hui Liu, 2011. The RAB38-Hosted Chicken Mir-1657 Gene: Sequence Conservation, Seed Region Polymorphisms and Putative Targets. Journal of Animal and Veterinary Advances, 10: 2343-2348.