Authors : Dongsheng He, Yanzong Zhao, Danping Su and Xiaoyun Niu
Abstract: PCR was developed to evaluate for its ability to simultaneously detect viral infections of swine. Specific primers were designed for each sub-type of porcine circovirus type 2 (PCV-2); porcine circovirus type 2a (PCV-2a) and porcine circovirus type 2b (PCV-2b). Each target produced specific amplicon with a size of 229 bp (PCV-2a) and 785 bp (PCV-2b). The assay was sensitive and specific in detecting the target agent in clinical specimens. In conclusion, the PCR has the potential to be useful for routine molecular diagnosis and epidemiology.
Dongsheng He, Yanzong Zhao, Danping Su and Xiaoyun Niu, 2011. Development of PCR for the Identification of Porcine Circovirus Type 2 (PCV-2) Genotype PCV-2a and PCV-2b. Journal of Animal and Veterinary Advances, 10: 2398-2401.