Abstract: The goal of the present study was to evaluate the serum metabolic profile of idiopathic emaciated buffaloes. A total number of 20 non-pregnant buffaloes were subjected to study. Out of them, 10 buffaloes had poor coat, poor general body condition score which constituted the emaciated group. The remained animals (10) were clinically healthy and kept as control group. There were no postmortem pathological affections in the internal organs of animals except loss of fat depot and decrease of skeletal muscle mass. Serum biochemical analysis revealed significant decreases in serum total proteins (p<0.01), albumin (p<0.05), phosphorus (p<0.05) and zinc (p<0.01) levels. It could be concluded that the cause of emaciation in the investigated buffaloes may be attributed to zinc and phosphorus deficiency.