Authors : Ruangyote Pilajun and Metha Wanapat
Abstract: An in vitro study was conducted to evaluate effect of coconut oil and Mangosteen Peel Powder (MPP) supplementation on methane production and methanogen population in rumen liquor of swamp buffalo. Completely randomized design was used with nine treatments including control and supplementation with coconut oil (3, 6% DM) and/or MPP (2, 4% DM). Supplementation of coconut oil and MPP significantly reduced methane production when compared with control group (p<0.05) however, increasing coconut oil and MPP levels decreased proportion of methane reduction. Population of methanogen was unchanged by supplementations (p>0.05) in contrast, the methanogen diversity slightly differed among treatments, especially when combinations with coconut oil and MPP used. The UPGMA dendrogram showed supplementation of coconut oil and MPP combination had pronounced effect on gel profile than that of single supplementation, especially combination of 6% of coconut oil with 2% of MPP which resulted in lowest band numbers. In addition, supplementation by high level of coconut oil or MPP did not show positive effects. Therefore, suitable level should not exceed than 6% for coconut oil and 4% DM for MPP supplementations, respectively.
Ruangyote Pilajun and Metha Wanapat , 2011. Methane Production and Methanogen Population in Rumen Liquor of Swamp Buffalo as Influenced by Coconut Oil and Mangosteen Peel Powder Supplementation. Journal of Animal and Veterinary Advances, 10: 2523-2527.