Abstract: The complete coding sequences of three sheep genes-ARF3, ARF4 and ARF5 were amplified using the Reverse Transcriptase Polymerase Chain Reaction (RT-PCR). The nucleotide sequences of these three genes revealed that sheep ARF3 gene encodes a protein of 181 amino acids that shares high homology with the ADP-Ribosylation Factor 3 (ARF3) proteins of five species human (99%), zebrafish (99%), African clawed frog (99%), pig (99%) and atlantic salmon (98%). The sheep ARF4 gene encodes a protein of 180 amino acids that shares high homology with the ADP-Ribosylation Factor 4 (ARF4) proteins of third species-cattle (100%), platypus (99%), green anole (99%), pig (99%), rabbit (98%), African clawed frog (98%), red jungle fowl (98%), mouse (98%), zebrafish (96%), human (97%), atlantic salmon (92%), rainbow trout (92%) and Caligus rogercresseyi (89%). The sheep ARF5 gene encodes a protein of 180 amino acids that shares high homology with the ADP-Ribosylation Factor 5 (ARF5) proteins of seven species human (100%), mouse (100%), rat (100%), gray short-tailed opossum (99%), chicken (98%), channel catfish (93%) and zebrafish (93%). Finally, these three novel sheep genes were assigned to GeneIDs; 100302303, 100302304 and 100302305. The phylogenetic analysis indicated that the sheep ARF3 gene has closer genetic relationship with the ARF3 gene of human. The sheep ARF4 gene has closer genetic relationship with the ARF4 gene of cattle and the sheep ARF5 gene has closer genetic relationship with the ARF5 genes of human, mouse and rat. Tissue expression profile analysis was also carried out and results demonstrated that sheep ARF3, ARF4 and ARF5 genes were differentially expressed in detected tissues.