Authors : Xin Wei, Wei Jian-Zhong, Huang Xiao-Hui, Yin Zong-Jun, Sun Pei and Li Yu
Abstract: To investigate the characteristics of serology and molecular epidemiology of Haemophilus parasuis in Anhui area, this research adopts KRG serotyping and ERIC-PCR and PCR-RFLP genotyping to conduct systematic analyses on 18 HPS isolates from Anhui. The result shows that 55.6% bacterial strains can be serotyped where the most prevalent serotype is type 4 (27.78%) followed by type 14 (16.67%) and type 13 (11.11%). About 44.4% bacterial strains cannot be serotyped. With ERIC-PCR analyses, 18 HPS isolates contain 4-9 bands with the size ranging from 100-2000 bp and they belong to 11 different genotypes where IV is the dominant genotype (27.78%) followed by type 2 (16.67%) and type 1 (11.11%). With PCR-RFLP analyses, 18 HPS isolates lie in 6 different genotypes where the most prevalent genotype in Anhui area is DBP (44.4%) followed by BAE (22.22%) and BBB (16.67%). The result revels that HPS prevails in pigs in Anhui area and both its serotypes and genotypes are in polymorphism distribution. The result also shows that KRG serotypes, ERIC-PCR genotypes and PCR-RFLP genotypes of HPS are not significantly associated with each other but ERIC-PCR genotype is associated with the sources of bacterial strains with the same serotype. Compared with PCR-RFLP, ERIC-PCR better uncovers the genetic relationships of bacterial strains and hence reveals the genetic distance among different bacterial strains which can be especially applied to the inter-pig and inter-farm epidemic conditions of pathogenium.
Xin Wei, Wei Jian-Zhong, Huang Xiao-Hui, Yin Zong-Jun, Sun Pei and Li Yu, 2011. The Research of KRG Serotyping and ERIC-PCR and PCR-RFLP Genotyping of Haemophilus parasuis Isolates from Anhui Province of China. Journal of Animal and Veterinary Advances, 10: 2669-2674.