Authors : Mahmoud R. Abd Ellah, Rofaida F. Abd-Elrahman, Hanaa G. Ahmed, Heba T. Khalaf, Reham R. Mahmoud, Shaimaa O. Sayed, Rehab M. Mahmoud, Rania M. Ali, Mona F. Abd-Elmotagaly, Ansaf N. Abd-Elhadi, Asmaa M. Abd-Elhamid, Ayat Kh. Mahmoud and Amany S. Abd-Elmegid
Abstract: The present study was undertaken to investigate the effect of storage time and temperature on erythrocytes, platelets and leucocytes pictures of cattle and equine blood. Whole blood samples from cows (No = 6) and donkeys (No = 6) were subdivided into two equal groups: refrigerator group (4°C) and room temperature group (30°C). Hematological analysis was performed by automatic blood cell counter at 0, 24, 30, 48, 56 and 72 h. The results revealed that donkey and cow’s blood showed insignificant changes in different hematological parameters kept at 4°C when compared with 0 h group. Storage at room temperature affected granulocytes counts only which significantly decreased at 48, 56 and 72 h in donkey’s blood and at 72 h in cow’s blood. In conclusion, it is possible to analyze blood samples kept refrigerated or at room temperature for erythrocytes and platelets picture within 3 days from blood collection in cattle and equines. On the other hand, granulocytes count must be carried out before 48 h from blood collection in donkeys and before 72 h in cow’s blood kept at room temperature.
Mahmoud R. Abd Ellah, Rofaida F. Abd-Elrahman, Hanaa G. Ahmed, Heba T. Khalaf, Reham R. Mahmoud, Shaimaa O. Sayed, Rehab M. Mahmoud, Rania M. Ali, Mona F. Abd-Elmotagaly, Ansaf N. Abd-Elhadi, Asmaa M. Abd-Elhamid, Ayat Kh. Mahmoud and Amany S. Abd-Elmegid, 2011. Effect of Storage Time and Temperature on Erythrocytes, Platelets and Leucocytes Pictures of Cattle and Equine Blood. Journal of Animal and Veterinary Advances, 10: 2768-2771.