Authors : H.A. Abbasi, S. Gharavysi and R. Abdullahpour
Abstract: Leptin a 16 kDa protein synthesized by white adipose tissue and involved in regulation of feed intake, energy balance, fertility and immune function. In order to evaluate the leptin gene receptor polymorphism the researchers have used a Restriction Fragment Length Polymorphism (RFLP) method. Blood samples were collected from randomly chosen 100 Mazandaran native fowls. Genomic DNA was extracted using modified salting-out method and used amplified polymerase chain reaction technique. Exon and intron 9-11 of the fowl leptin gene receptor was amplified to produce a 382 bp fragment. The PCR products were electrophoresed on 1% agarose gel and stained by etidium bromide. Then, they were digested of amplicons with Tsp509I and revealed two alleles A and B. Data were analysed using PopGene 32 package. In this population, AA, AB, BB genotype have been identified with the 69.14, 30.16, 0.7% frequencies. A and B alleles frequencies were 0.84, 0.16, respectively. The χ2-test did not signify Hardy-Weinberg equilibrium in this population (p<0.05). Further association analysis is required to clarify the effects of these marker genotypes on production traits in this breeder flock.
H.A. Abbasi, S. Gharavysi and R. Abdullahpour , 2011. Genetic Polymorphism Exon 9-11 at the Leptin Gene Receptor in Breeder Hens of Mazandaran Native Fowls. Journal of Animal and Veterinary Advances, 10: 2802-2805.