Authors : Wen-Ming Zhao, Rong-Xue Zhao, Na Qiao, Qi Xu, Zheng-Yang Huang, Xiu Li, Yang Zhang and Guo-Hong Chen
Abstract: In this study, the exons polymorphism of Growth Hormone (GH) gene were detected by Polymerase Chain Reaction-Single Strand Conformation Polymorphism (PCR-SSCP) and DNA sequencing method in 109 individuals from Shitou goose, Wanxi white goose and Zi goose. About 5 pairs of primer were designed according to the GH gene sequence of goose then the association of polymorphism of GH gene with the growth traits was studied. The results indicated there were two nucleotide mutations at P2 and P4 locus, respectively which resulted in 10 genotypes and 3 genotypes. The χ2-test showed that the genotype distributions of GH gene were in agreement with Hardy-Weinberg equilibrium in P2 and P4 locus (p>0.05), the difference was extremely significant in P2 locus on the distribution of genotype for Shitou goose and Zigoose while for Shitou goose, Wanxi white goose and Zi goose, it was extreme significant at P4 locus but there was no difference for the other locus. The least square on genotypes and groups analysis showed that there were significant differences (p<0.01) on growth traits in different groups however, only P4 locus have genotypes effect, the average value of AA genotype was the highest therefore, AA genotype could be the favorable marker for early breeding selection for high product trait of local goose.
Wen-Ming Zhao, Rong-Xue Zhao, Na Qiao, Qi Xu, Zheng-Yang Huang, Xiu Li, Yang Zhang and Guo-Hong Chen, 2011. Association of GH Polymorphisms with Growth Traits in Goose. Journal of Animal and Veterinary Advances, 10: 692-697.