Authors : Zhicai Zuo, Shan Liao, Xinqiao Qi, Yue Yi, Zhiwen Xu, Ling Zhu, Wanzhu Guo and Hengmin Cui
Abstract: BCL-10 is an important functional and an apoptotic regulator gene inducing the organismic innate immunity. It has not been reported that the BCL-10 protein of porcine in normal level could motivate immune factor to produce. Bioinformatics analysis was performed to predict the characteristics of BCL-10, the results indicated that BCL-10 gene encoded a polypeptide, molecular mass of 45 Ku and comprising 233 amino acids. The prediction of secondary structure showed that BCL-10 had 9 alpha-helix, 1 beta-sheet, 12 turn and 14 random coil. The protein had twenty transmembrane regions, an CARD structure field which located in 15-100 amino acids and no signal peptide, the hydrophobic index was between -2.500 and 1.400. The phylogenetic tree showed that BCL-10 was similar to human (the homology index was >95%) and had a distant relationship to mouse. To identify the activity of BCL-10, the protein with a His-tag was expressed in E. coli Rosetta and purified. The purified protein showed an obvious effect of cell proliferation. Cloning and analysising of BCL-10 gene which laid foundation to further research and exploitation.
Zhicai Zuo, Shan Liao, Xinqiao Qi, Yue Yi, Zhiwen Xu, Ling Zhu, Wanzhu Guo and Hengmin Cui, 2012. Prokaryotic Expression and Sequence Analysis of Porcine BCL10 Gene. Journal of Animal and Veterinary Advances, 11: 1046-1051.