Authors : Yongmei Su, Wenjin Zhu and Jianhua Wu
Abstract: This experiment was conducted to study the polymorphism on intron 3 of GH gene in donkey in China. The polymorphism was analysised by PCR-SSCP in the 10 donkey breeds (JN, GL, GZ, DZ, JM, BY, QY, LZ, TH and XJ) by PCR-SSCP. One SNPs was found in the sequence of GH gene through the sequencing analysis of the two homozygous genes AA and BB, the mutation at 100th site (G→T) of the third intron of donkey GH gene was showed. Three genotypes AA, AB and BB were detected in 10 donkey breeds and the allele A was the predominant allele. All the breeds were in Hardy-Weinberg equilibrium at this polymorphic site (p>0.05). The results confirmed that there were polymorphisms in the intron 3 of GH gene.
Yongmei Su, Wenjin Zhu and Jianhua Wu, 2012. Polymorphism Analysis on Intron 3 of the GH Gene in Chinese Donkey. Journal of Animal and Veterinary Advances, 11: 1705-1707.