Authors : Niorn Ratanapob and Theera Rukkwamsuk
Abstract: Caprine Arthritis Encephalitis Virus (CAEV) is an important pathogen in goats. Definitive diagnosis depends on viral isolation and this method is beneficial for studying CAEV infection. Synovial fibroblast is the most appropriate cell for CAEV isolation. The objectives of this study were to culture synovial membrane cells and to identify whether the cultured cells were fibroblast. Synovial membrane tissue was collected from a CAEV-seronegative, 5 months old, male goat. The tissue was digested by collagenase. Synovial cells were cultured in RPMI 1640 at 37°C and 5% carbon dioxide. The cultured synovial cells were detected for vimentin, a marker for fibroblastic cell type, using immunocytochemistry. Phagocytic activity of the synovial cells was also tested by adding carbon particle in culture media to confirm whether or not the cells were synovial macrophages. Results revealed that the cultured synovial cells were spindle-shaped and were attached to the bottom of the flasks. These primary cultured cells could be subcultured >9 passages. Vimentin could be detected but phagocytic activity could not be detected in these cultured synovial cells indicating that these cultured cells were fibroblast.
Niorn Ratanapob and Theera Rukkwamsuk, 2012. Culture and Identification of Goat Synovial Membrane Cells for aprine Arthritis Encephalitis Virus Diagnosis. Journal of Animal and Veterinary Advances, 11: 1827-1830.