Authors : Sun Quan-Wen, Wu Shu-Qin, Yang Cui-Jun, Wang Jin-Liang, Ge Jian, Chang Bao and Xu Tong
Abstract: To develop a new, rapid, practical and sensitive method for detection of Porcine Epidemic Diarrhea Virus (PEDV), a Reverse Transcription Loop Mediated isothermal Amplification (RT-LAMP) assay was established based on six primers in the conserved gene. The method had specificity but no cross-reaction with other viruses and was 100 fold higher than RT-PCR. The result suggested that the newly developed RT-LAMP assay is a simple and specific method for rapid detection of PEDV in field conditions and it has a high practical value in PEDV integrated control and early diagnosis.
Sun Quan-Wen, Wu Shu-Qin, Yang Cui-Jun, Wang Jin-Liang, Ge Jian, Chang Bao and Xu Tong, 2012. Development of a Reverse Transcription Loop-Mediated Isothermal Amplification Assay for Visual Detection of Porcine Epidemic Diarrhea Virus. Journal of Animal and Veterinary Advances, 11: 1897-1900.