Authors : Yongfeng Sun, Tongao Yang, Jingtao Hu, Zhe Hao, Yujian Sui, Yingying Fu, Lu Chen, Haiyang Zhu and Wei Wu
Abstract: The muscular tissue of breast was dissected from 8 weeks old Jilin White goose in the present study. The big fragment PCR Method was used to amplify double-strand cDNA based on the SMART techniques for construction of a full-length cDNA library. After digestion with restriction endonuclease Sfi |, a modified vector of pBluescript II SK-plasmid with the adaptors containing Sfi |A and Sfi |B sites was used to recombine with the cDNA products amplified. The recombinants were cloned by transformation into competent Escherichia coli DH2α. A plasmid cDNA library with goose muscle was constructed. The results showed that the titer of the cDNA library was 1.01x106 pfu mL-1 and the percentage of recombinant clones was 97%. The length of most cDNA inserted was between 0.25 and 1.6 kb identified by gel electrophoresis after cDNA PCR amplification. The unigene ratio was 66.7% and the percentage of complete cDNA sequences was 80% by estimating from the 24 clones sequenced randomly. It is helpful to study muscle development of goose at molecular level in the future.
Yongfeng Sun, Tongao Yang, Jingtao Hu, Zhe Hao, Yujian Sui, Yingying Fu, Lu Chen, Haiyang Zhu and Wei Wu, 2012. Construction of Full-Length Goose Muscle cDNA Library. Journal of Animal and Veterinary Advances, 11: 2106-2109.