Authors : Xueqing Liu, Hao Yang, Jiayin Peng, Qiuju Chen and Binyun Cao
Abstract: Isolation and culture of mammary epithelial cells in vitro is of great significance for further study of the mechanism of lactation, verification of mammary gland expression vector and preparation of mammary gland bioreactor. In this study, in order to obtain an efficient method to culture Goat Mammary Gland Epithelial (GMGE) cells with high purity in vitro, researchers successfully constructed a new method called Tissue Re-Shift Method. About 5 methods which include Tissue Explant Culture Method, Tissue Re-Shift Method, Trypsin Digestion Method, Collagenase Digestion Method and the Combination of Collagenase digestion with Tissue Adherence Method were applied and compared. In spite of no epithelial cells cultured by Trypsin Digestion Method and low capability of proliferation by combination of collagenase digestion with Tissue Adherence Method, a considerable number of GMGE cells were collected by another 3 methods. By the detection of immunofluorescence, Tissue Re-Shift Method can obtain pure GMGE cells in the shortest time. Biological characteristics of GMGE cells isolated from above 3 methods were evaluated by cell population doubling time and cell growth curves. The results indicated that cell growth curves of 3 methods showed typical S type and GMGE cells isolated from Tissue Re-Shift Method had maximum proliferative capacity. Finally, the existence of the β-casein verified that they still had the high ability of lactation in the primary culture. In conclusion, Tissue Re-Shift Method was an ideal way to obtain GMGE cells for further study.
Xueqing Liu, Hao Yang, Jiayin Peng, Qiuju Chen and Binyun Cao, 2012. Establishment and Evaluation of a New Method Tissue Re-Shift for Isolation of Goat Mammary Epithelial Cells. Journal of Animal and Veterinary Advances, 11: 2403-2408.