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Journal of Animal and Veterinary Advances
Year: 2012 | Volume: 11 | Issue: 2 | Page No.: 245-251
DOI: 10.3923/javaa.2012.245.251  
Molecular Cloning, Sequence Identification and Tissue Expression Profile of Three Novel Sheep (Ovis aries) Genes-ZFAND5, ZGPAT and ZDHHC7
Liu Yonggang and Xia Xueshan
 
Abstract: The complete coding sequences of three sheep genes ZFAND5, ZGPAT and ZDHHC7 were amplified using the Reverse Transcriptase Polymerase Chain Reaction (RT-PCR). The sheep ZFAND5 gene encodes a protein of 213 amino acids that shares high homology with the Zinc finger, AN1-type Domain 5 (ZFAND5) proteins of eight species; cattle (100%), pig (99%), human (99%), rabbit (99%), mouse (98%), giant panda (98%), gray short-tailed opossum (97%) and Northern white-cheeked gibbon (97%). The sheep ZGPAT gene encodes a protein of 513 amino acids that shares high homology with the Zinc finger CCCH-type with G patch domain-containing protein (ZGPAT) proteins of seven species; cattle (97%), giant panda (84%), rabbit (99%), human (79%), rat (76%), mouse (77%) and chimpanzee (78%). The sheep ZDHHC7 gene encodes a protein of 308 amino acids that shares high homology with the Zinc finger, DHHC-type containing 7 (ZDHHC7) proteins of nine species; cattle (99%), dog (93%), pig (93%), human (93%), giant panda (93%), Northern white-cheeked gibbon (92%), mouse (92%), white-tufted-ear marmoset (92%) and rat (92%). Finally, these three novel sheep genes were assigned to GeneIDs; 100302558, 100302028 and 100302557. The phylogenetic analysis revealed that the sheep ZFAND5, ZGPAT and ZDHHC7 genes all have a closer genetic relationship with the ZFAND5, ZGPAT and ZDHHC7 genes of cattle. Tissue expression profile analysis was also carried out and results demonstrated that sheep ZFAND5, ZGPAT and ZDHHC7 genes were all generally but differentially expressed in detected tissues.
 
How to cite this article:
Liu Yonggang and Xia Xueshan, 2012. Molecular Cloning, Sequence Identification and Tissue Expression Profile of Three Novel Sheep (Ovis aries) Genes-ZFAND5, ZGPAT and ZDHHC7. Journal of Animal and Veterinary Advances, 11: 245-251.
DOI: 10.3923/javaa.2012.245.251
URL: http://medwelljournals.com/abstract/?doi=javaa.2012.245.251