Authors : Alper Baran, Ozen Banu Ozdas, Asiye Izem Sandal and Kemal Ak
Abstract: This study was aimed to compare the effects of freezing dog semen in straws in 0.1% fat bearing Skim Milk-Glucose (SMG) extender with the routinely used TRIS-Fructose-Citric acid (TFC) extender regarding the post-thaw motility, acrosome and total morphologic defects rate. Four German shepherd male dogs of 2-3 years under the same managemental conditions on a private kennel were used. Six ejaculates per each dog (total 24 ejaculates) were collected digital manipulation. Two extenders used were 20% egg yolk containing TFC and 10% egg yolk containing SMG extenders. Semen was divided into two split parts and one was extended with TFC and the other with SMG extenders at 26°C at a rate 1:1 and cooled to 5°C in an hour. Cooled semen samples were further extended with 10% (v/v) glycerol containing extender (for an hour) at equal rates (final glycerol rate 5%). Following glycerolisation semen samples were filled into 0.5 mL straws and equilibrated for an hour at 5°C. Straws were frozen at –110°C liquid nitrogen vapour for 7 min. Labeled straws were stored in separate canisters in liquid nitrogen at –196°C. Six straws for TFC extender and six straws for SMG extender were stored to be examined. Straws were thawed at 45°C for 60 sec. Post-thaw motility rate of TFC and SMG extended semen samples were, respectively 48.54±8.27 and 51.97±7.51%. Acrosome and total morphologic defect rates were 41.04±9.44 and 51.17±9.05% for TFC; 38.04±13.60 and 46.67±12.68% for SMG extender, respectively. It has been founded that when the post-thaw sperm traits of straws frozen dog semen was evaluated, SMG extender have been successful at least as much as TFC extender.
Alper Baran, Ozen Banu Ozdas, Asiye Izem Sandal and Kemal Ak, 2012. Effects of Skim Milk and Tris Extender on Frozen-Thawed Canine Sperm Morphology. Journal of Animal and Veterinary Advances, 11: 3242-3246.